primary antibodies against p21  (Thermo Fisher)

Journal: Scientific Reports

Article Title: Vibrational spectroscopy unveils distinct cell cycle features of cancer stem cells in melanoma

doi: 10.1038/s41598-025-14018-8

Figure Lengend Snippet: Immunohistochemical staining of p16 and p21 expression in CHL-1 melanoma cells, CD133 + melanoma stem-like cells, and CD133- non-stem melanoma cells at different time points (11 h, 24 h, 48 h, and 72 h). Representative images show the time-dependent expression of p16 and p21 in each cell type, with higher magnification insets. Scale bars: 100 μm. Bar graphs on the right represent the quantification of p16- and p21-positive cells (mean ± SD) for each group at the indicated time points.

Article Snippet: Primary antibodies against p21 (Bioss, BB07142233) and p16 (Bioss, BB10114929) were diluted at 1:200 and applied to the cells.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Aging Cell

Article Title: Mechanistic Insights Into 5′‐ tiRNA ‐His‐ GTG Mediated Activation of the JNK Pathway in Skin Photoaging

doi: 10.1111/acel.70049

Figure Lengend Snippet: Overexpression of 5′‐tiRNA‐His‐GTG in HDF cells induced cellular senescence. (A) qRT‐PCR validation of the overexpression of 5′‐tiRNA‐His‐GTG in HDF cells. (B) Overexpressing 5′‐tiRNA‐His‐GTG in HDF cells and staining with SA‐β‐gal after 24 h. Cells with blue staining represent senescent cells. The treated cells were divided into three groups: unstained, strongly positive, and weakly positive. The percentage of cells in each group was presented in a bar graph. The scale bar, 100 μm. (C) WB analysis of Collagen Type I, p53, and p21 in HDF cells after 5′‐tiRNA‐His‐GTG Mimic transfection. (D) The mRNA levels of IL ‐ 1β , IL ‐ 6 , and IL ‐ 8 in HDF cells after 5′‐tiRNA‐His‐GTG Mimic transfection. (E) EdU assay was used to analyze HDF cells' proliferation ability after overexpressing 5′‐tiRNA‐His‐GTG. The scale bar is 100 μm.

Article Snippet: Primary antibodies against p21 (10355‐1‐AP), p53 (10442‐1‐AP), and Collagen type I polyclonal antibody (14695‐1‐AP) were purchased from Proteintech (China).

Techniques: Over Expression, Quantitative RT-PCR, Biomarker Discovery, Staining, Transfection, EdU Assay

Journal: Aging Cell

Article Title: Mechanistic Insights Into 5′‐ tiRNA ‐His‐ GTG Mediated Activation of the JNK Pathway in Skin Photoaging

doi: 10.1111/acel.70049

Figure Lengend Snippet: Inhibiting 5′‐tiRNA‐His‐GTG rescued UVB‐induced HDF cells photoaging. (A) HDF cells were exposed to UVB after inhibiting 5′‐tiRNA‐His‐GTG, and stained with SA‐β‐gal after 24 h. Cells with blue staining represent senescent cells. The treated cells were divided into three groups: unstained, strongly positive, and weakly positive. The percentage of cells in each group was presented in a bar graph. The scale bar, 100 μm. (B) WB analysis of Collagen Type I, p53, and p21 in the photoaging cell model after 5′‐tiRNA‐His‐GTG Inhibitor transfection. (C) The mRNA levels of IL ‐ 1β , IL ‐ 6 , and IL ‐ 8 in the photoaging cell model after 5′‐tiRNA‐His‐GTG Inhibitor transfection. (D) EdU assay was used to analyze cell proliferation ability in the photoaging cell model after 5′‐tiRNA‐His‐GTG Inhibitor transfection. The scale bar, 100 μm.

Article Snippet: Primary antibodies against p21 (10355‐1‐AP), p53 (10442‐1‐AP), and Collagen type I polyclonal antibody (14695‐1‐AP) were purchased from Proteintech (China).

Techniques: Staining, Transfection, EdU Assay

Journal: Aging Cell

Article Title: Mechanistic Insights Into 5′‐ tiRNA ‐His‐ GTG Mediated Activation of the JNK Pathway in Skin Photoaging

doi: 10.1111/acel.70049

Figure Lengend Snippet: Overexpression of NUP98 rescues 5′‐tiRNA‐His‐GTG‐induced cell senescence in HDF cells. (A) WB analysis of oe‐NUP98 in HDF cells. (B) WB analysis of Collagen Type I, p53 and p21 in HDF cells and oe‐NUP98 HDF cells, with 5′‐tiRNA‐His‐GTG Mimic transfection. (C) The mRNA levels of IL ‐ 1β , IL ‐ 6 and IL ‐ 8 in HDF cells and oe‐NUP98 HDF cells, after 5′‐tiRNA‐His‐GTG Mimic transfection. (D) EdU assay was used to analyze HDF cells and oe‐NUP98 HDF cells proliferation ability after 5′‐tiRNA‐His‐GTG Mimic transfection. The scale bar, 100 μm. (E) HDF cells and oe‐NUP98 HDF cells were transfected with 5′‐tiRNA‐His‐GTG Mimic and stained with SA‐β‐gal after 48 h. Cells with blue staining represent senescent cells. The treated cells were divided into three groups: unstained, strongly positive, and weakly positive. The percentage of cells in each group was presented in a bar graph. The scale bar, 100 μm.

Article Snippet: Primary antibodies against p21 (10355‐1‐AP), p53 (10442‐1‐AP), and Collagen type I polyclonal antibody (14695‐1‐AP) were purchased from Proteintech (China).

Techniques: Over Expression, Transfection, EdU Assay, Staining

Journal: Aging Cell

Article Title: Mechanistic Insights Into 5′‐ tiRNA ‐His‐ GTG Mediated Activation of the JNK Pathway in Skin Photoaging

doi: 10.1111/acel.70049

Figure Lengend Snippet: Overexpression of NUP98 attenuates cellular senescence. (A) oe‐NUP98 HDF cells were stained with SA‐β‐gal after 48 h. Cells with blue staining represent senescent cells. The treated cells were divided into three groups: unstained, strongly positive, and weakly positive. The percentage of cells in each group was presented in a bar graph. The scale bar is 100 μm. (B) The mRNA levels of IL ‐ 1β , IL ‐ 6 and IL ‐ 8 in oe‐NUP98 HDF cells. (C) WB analysis of NUP98, Collagen Type I, p53 and p21 in oe‐NUP98 HDF cells. (D) EdU assay was used to analyze oe‐NUP98 HDF cells' proliferation ability. The scale bar is 100 μm.

Article Snippet: Primary antibodies against p21 (10355‐1‐AP), p53 (10442‐1‐AP), and Collagen type I polyclonal antibody (14695‐1‐AP) were purchased from Proteintech (China).

Techniques: Over Expression, Staining, EdU Assay